Boronate affinity saccharide electrophoresis: A novel carbohydrate analysis tool
Identifieur interne : 001401 ( Main/Exploration ); précédent : 001400; suivant : 001402Boronate affinity saccharide electrophoresis: A novel carbohydrate analysis tool
Auteurs : Thomas R. Jackson [Royaume-Uni] ; Jeremy S. Springall [Royaume-Uni] ; Damien Rogalle [Royaume-Uni] ; Naoko Masumoto [Royaume-Uni] ; Hung Ching Li [Royaume-Uni] ; François D'Hooge [Royaume-Uni] ; Semali P. Perera [Royaume-Uni] ; Toby A. Jenkins [Royaume-Uni] ; Tony D. James [Royaume-Uni] ; John S. Fossey [Royaume-Uni] ; Jean M. H. Van Den Elsen [Royaume-Uni]Source :
- ELECTROPHORESIS [ 0173-0835 ] ; 2008-11.
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Abstract
The incorporation of specialised carbohydrate affinity ligand methacrylamido phenylboronic acid in polyacrylamide gels for fluorophore‐assisted carbohydrate electrophoresis greatly improved the effective separation of saccharides that show similar mobilities in standard electrophoresis. Polyacrylamide gel electrophoresis using methacrylamido phenylboronic acid in low loading (typically 0.5–1% dry weight) was unequivocally shown to alter retention of labelled saccharides depending on their boronate affinity. While conventional fluorophore‐assisted carbohydrate electrophoresis of 2‐aminoacridone labelled glucose oligomers showed an inverted parabolic migration, an undesired trait of small oligosaccharides labelled with this neutral fluorophore, boron affinity saccharide electrophoresis separation of these carbohydrates completely restored their predicted running order, based on their charge/mass ratio, and resulted in improved separation of the analyte saccharides. These results exemplify boron affinity saccharide electrophoresis as an important new technique for analysing carbohydrates and sugar‐containing molecules.
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DOI: 10.1002/elps.200800178
Affiliations:
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Polyacrylamide gel electrophoresis using methacrylamido phenylboronic acid in low loading (typically 0.5–1% dry weight) was unequivocally shown to alter retention of labelled saccharides depending on their boronate affinity. While conventional fluorophore‐assisted carbohydrate electrophoresis of 2‐aminoacridone labelled glucose oligomers showed an inverted parabolic migration, an undesired trait of small oligosaccharides labelled with this neutral fluorophore, boron affinity saccharide electrophoresis separation of these carbohydrates completely restored their predicted running order, based on their charge/mass ratio, and resulted in improved separation of the analyte saccharides. These results exemplify boron affinity saccharide electrophoresis as an important new technique for analysing carbohydrates and sugar‐containing molecules.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Midlands de l'Ouest</li></region><settlement><li>Birmingham</li></settlement><orgName><li>Université de Birmingham</li></orgName></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Jackson, Thomas R" sort="Jackson, Thomas R" uniqKey="Jackson T" first="Thomas R." last="Jackson">Thomas R. Jackson</name></noRegion><name sortKey="Ching Li, Hung" sort="Ching Li, Hung" uniqKey="Ching Li H" first="Hung" last="Ching Li">Hung Ching Li</name><name sortKey="D Hooge, Francois" sort="D Hooge, Francois" uniqKey="D Hooge F" first="François" last="D'Hooge">François D'Hooge</name><name sortKey="Fossey, John S" sort="Fossey, John S" uniqKey="Fossey J" first="John S." last="Fossey">John S. Fossey</name><name sortKey="Fossey, John S" sort="Fossey, John S" uniqKey="Fossey J" first="John S." last="Fossey">John S. Fossey</name><name sortKey="Fossey, John S" sort="Fossey, John S" uniqKey="Fossey J" first="John S." last="Fossey">John S. Fossey</name><name sortKey="James, Tony D" sort="James, Tony D" uniqKey="James T" first="Tony D." last="James">Tony D. James</name><name sortKey="Jenkins, Toby A" sort="Jenkins, Toby A" uniqKey="Jenkins T" first="Toby A." last="Jenkins">Toby A. Jenkins</name><name sortKey="Masumoto, Naoko" sort="Masumoto, Naoko" uniqKey="Masumoto N" first="Naoko" last="Masumoto">Naoko Masumoto</name><name sortKey="Perera, Semali P" sort="Perera, Semali P" uniqKey="Perera S" first="Semali P." last="Perera">Semali P. Perera</name><name sortKey="Rogalle, Damien" sort="Rogalle, Damien" uniqKey="Rogalle D" first="Damien" last="Rogalle">Damien Rogalle</name><name sortKey="Springall, Jeremy S" sort="Springall, Jeremy S" uniqKey="Springall J" first="Jeremy S." last="Springall">Jeremy S. Springall</name><name sortKey="Van Den Elsen, Jean M H" sort="Van Den Elsen, Jean M H" uniqKey="Van Den Elsen J" first="Jean M. H." last="Van Den Elsen">Jean M. H. Van Den Elsen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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